Evaluation of limulus amebocyte lysate and recombinant endotoxin alternative assays for an assessment of endotoxin detection specificity
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چکیده
منابع مشابه
Evaluation of the Limulus amebocyte lysate and recombinant factor C assays for assessment of airborne endotoxin.
As a potent inflammatory agent, endotoxin is a key analyte of interest for studies of lung ailments in domestic environments and occupational settings with organic dust. A relatively unexplored advance in endotoxin exposure assessment is the use of recombinant factor C (rFC) from the Limulus pathway in a fluorometric assay. In this study, we compared airborne endotoxin concentrations in laborat...
متن کاملSingle-step, chromogenic Limulus amebocyte lysate assay for endotoxin.
A new reagent for the chromogenic Limulus amebocyte lysate (LAL) assay is described. LAL was formulated for optimal performance in either an endpoint procedure or a kinetic procedure with the chromogenic substrate, buffer, and LAL components colyophilized as a single reagent. The kinetic chromogenic method required an incubating microplate reader coupled to a computer for collection and analysi...
متن کاملEndotoxin Contamination of Large Volume Parenterals as Detected by Limulus Amebocyte Lysate as an Alternative to Rabbit Pyrogen Test
The possibility of replacing the rabbit pyrogen test by the Limulus Amebocyte lysate (LAL) test, as a final release test for Large Volume Parenterals (LVPs) was investigated. The sensitivity and specificity of the LAL test and rabbit pyrogen assay were studied by means of artificially contaminated parenterals. Various LVPs (mostly containing electrolytes) were spiked with 0.5 and 5 EUlml o...
متن کاملMeasuring of free endotoxin in alum-precipitated vaccine of haemorrhagic septicaemia by limulus amebocyte lysate test
Haemorrhagic septicaemia (HS) vaccine which is prepared in Razi Institute is used in endemic areas ofIran. Aluminum-hydroxide gel was used as adjuvant for preparing this vaccine. Post-vaccinal shock reactionswere the main complaint after use of this vaccine. In a previous study, we could improve the vaccine byalum-precipitation Pasteurella multocida cells and removing the liquid phase. In this ...
متن کاملendotoxin contamination of large volume parenterals as detected by limulus amebocyte lysate as an alternative to rabbit pyrogen test
the possibility of replacing the rabbit pyrogen test by the limulus amebocyte lysate (lal) test, as a final release test for large volume parenterals (lvps) was investigated. the sensitivity and specificity of the lal test and rabbit pyrogen assay were studied by means of artificially contaminated parenterals. various lvps (mostly containing electrolytes) were spiked with 0.5 and 5 eulml of e.c...
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ژورنال
عنوان ژورنال: European Journal of Pharmaceutical Sciences
سال: 2021
ISSN: 0928-0987
DOI: 10.1016/j.ejps.2021.105716